World Practical Course

European Molecular Biology Organization

Structure and Dynamics of

Biomolecules by NMR Spectroscopy

 

 

 

 

 



21 - 30 September | 2009 |Rosario| Argentina

Programme (outline)

 

   

Lectures (L1-L20)

  1. Introductory Lecture: NMR past & future (Griesinger)
  2. Heteronuclear NMR: basic tools & tricks I (Fernández)
  3. Heteronuclear NMR: basic tools & tricks II (Sattler)
  4. Phase cycles and gradients in NMR experiments (Sattler)
  5. Labeling approaches for protein NMR studies (Almeida)
  6. Ab initio chemical shifts calculations (Case)
  7. J-couplings (Vuister)
  8. Data Processing and analysis (Delaglio)
  9. Relaxation (Palmer)
  10. TROSY, cross-correlated relaxation (Griesinger)
  11. Residual dipolar couplings I (Zweckstetter)
  12. Residual dipolar couplings II (Tolman)
  13. Relaxation & Residual dipolar couplings (Blackledge)
  14. Structure Calculation: Angle & distance restraints (Güntert)
  15. NMR of Paramagnetic Molecules (Vila)
  16. Paramagnetic Constraints in NMR (Luchinat)
  17. Conformation and dynamics of non-native proteins (Blackledge)
  18. NMR of nucleic acids (Carlomagno)
  19. NMR of Large Proteins (Sattler)
  20. Validation of protein structures derived by NMR (Vuister)
  

Practicals (P1-P14)

1)         NMR: Pulse calibration, basic experiments (Local staff)
2)         NMR: Triple Resonance Experiments (Local staff)
3)         Data processing and analysis: CARA (Local staff)
4)         Data processing and analysis: NMR Pipe (Delaglio)
5)         Structure calculation: X-Plor (Parigi)
6)         Structure calculation: Cyana (Güntert)
7)         Residual dipolar couplings: sample preparation and measurements (Local staff, Tolman)  
8)         Residual dipolar couplings: PALES (Zweckstetter)
9)         Residual dipolar couplings and 15N relaxation: Module/Tensor (Blackledge)
10)       15N Relaxation: ModelFree analysis (Palmer)
11)       NMR Prediction and Homology Tools: TALOS, SPARTA, MFR (Delaglio)
12)       Structure Validation: WHAT IF  (Vuister)
13)       Triple Resonance Experiments: HNCO from scratch (Carlomagno)
14)       Data processing and analysis: SPARKY (Local staff)



 

* A coffe break will be held mid-morning and mid-afternoon each day. 		 
   

 

  
   

 

 

 

 

 

 


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