Programme (Outline)
The course will take place over 11 working days with:
Week 1 (Mon-Sat)
Week 2 (Mon-Fri)
(The Sunday in between as a rest day)
The course will be composed of:
20% demonstrations and tutorials
30% seminars
50% practicals
The structure of the typical day will be 3 seminars in the morning, followed by an overview of the practicals, and then the practicals themselves in the afternoon. The evening will be open for participants to carry-out extra optional work on the instruments.
Participants will be encouraged to bring their own samples for investigation.
Week One
Will start with a mini-symposium (Day 1) where students will present their research topic of interest.
Day 2 will focus on basic microscopy, the anatomy of a microscope, and widefield fluorescence microscopy.
Days 3-6 will then offer four different practicals on rotation:
(i) line scanning confocal microscopy
(ii) spinning disk confocal microscopy
(iii) image processing and deconvolution
(iv) SPIM
Thus the first week will cover fundamental aspects of microscopy as well as 3-D imaging.
Week Two
Will concentrate on the F-techniques.
Each afternoon (Day 7-10) the participants will be taken on rotation through four different practicals:
(i) FRET by acceptor photobleaching
(ii) FRET by FLIM
(iii) FCS and FCCS
(iv) FRAP
The course will end (Day 11) with a dedicated session where participants can work on their own samples.
However, we will make a point to help participants image their own samples throughout the course as time allows.
Finally there will be an overview of the course and participants will be asked to comment on the course content and utility.
Further programme details will follow shortly.