World Practical Course

European Molecular Biology Organization


FRET, FLIM, FCS, FRAP & 3-D Imaging;

Application to Cell and Developmental Biology


 

 

 

 

 



13 - 24 April| 2009 | Institute of Medical Biology (IMB)| Singapore

About the Practical Course

 

The major objective of the course is to teach topics in basic and advanced microscopy to Ph. D students/Post-docs
with research interests in cell and developmental biology.

Of particular focus for the course are: quantitative fluorescence methods,  the F-techniques and modes of imaging in 3-D.

 

 
Topics will include:
 

Basic, widefield, and confocal microscopy (line scanning as well as spinning disc)

 

3-D imaging

 

Selective plane illumination microscopy (SPIM)

 

Fluorescence Correlation Spectroscopy (FCS)

 

Fluorescence Lifetime Imaging Microscopy (FLIM)

 

Fluorescence Recovery After Photobleaching (FRAP)

 

The application of these techniques to detect protein-protein interactions by Forster Resonance Energy Transfer (FRET).

 
Also featured in the course are the application of these imaging techniques to model cell and  developmental biology systems;
Mammalian cells, Yeast, Zebrafish and Drosophila. 

 

Co-Sponsors

 
             

 

European Molecular Biology Organization | Meyerhofstrasse 1 | 69117 Heidelberg | Germany