World Practical Course

European Molecular Biology Organization

FRET, FLIM, FCS, FRAP & 3-D Imaging;

Application to Cell and Developmental Biology






13 - 24 April| 2009 | Institute of Medical Biology (IMB)| Singapore

About the Practical Course


The major objective of the course is to teach topics in basic and advanced microscopy to Ph. D students/Post-docs
with research interests in cell and developmental biology.

Of particular focus for the course are: quantitative fluorescence methods,  the F-techniques and modes of imaging in 3-D.


Topics will include:

Basic, widefield, and confocal microscopy (line scanning as well as spinning disc)


3-D imaging


Selective plane illumination microscopy (SPIM)


Fluorescence Correlation Spectroscopy (FCS)


Fluorescence Lifetime Imaging Microscopy (FLIM)


Fluorescence Recovery After Photobleaching (FRAP)


The application of these techniques to detect protein-protein interactions by Forster Resonance Energy Transfer (FRET).

Also featured in the course are the application of these imaging techniques to model cell and  developmental biology systems;
Mammalian cells, Yeast, Zebrafish and Drosophila. 





European Molecular Biology Organization | Meyerhofstrasse 1 | 69117 Heidelberg | Germany