Programme

09:00 - 09:30

Opening session

Prof. Guillermo Dighiero:
Director of the Institut Pasteur de Montevideo

Dr. Martina Crispo
(co-organizer Institut Pasteur de Montevideo)

Drs. Jean-Louis Guénet/Jean-Jacques Panthier

(co-organizers Institut Pasteur Paris)

09:30 - 11:00

Lecture:
A century of mouse genetics - Origin and diversity of laboratory strains
(Jean - Louis Guénet)

11:00 - 11:30

Coffee break

11:30 - 12:30

Lecture:
Elements in formal genetics – Part 1
(Jean-Jacques Panthier)

 12:30 - 14:00

Lunch

14:00 - 16:30

Practical session:
(Supervised by Fernando Benavides)
Mouse necropsy, sampling for histopathology - Basics of mouse pathology

16:30 - 18:00

Lecture:
Genetic monitoring of inbred strains and outbred stocks. (Fernando Benavides)

From 18:00

Informal get-together

Tuesday 25th November

09:00 - 10:30

Lecture:
The different categories of genetically standardized laboratory strains (outbred and randombred stocks - inbred strains and their derivatives - congenic and recombinant inbred strains)
(Jean-Louis Guénet)

10:30 - 11:00

11:00 - 12:30

Practical session:
(supervised by Fernando Benavides, Jean-Jacques Panthier
and Xavier Montagutelli )

Genetic monitoring of inbred strains using microsatellites.          

1) Preparation of PCR and agarose gels

12:30 - 13:30

Lunch

13:30 - 15:00

Lecture:
Elements in formal genetics – Part 2
(Jean-Jacques Panthier)

15:00 - 18:00

Practical session:
(supervised by Fernando Benavides, Jean-Jacques Panthier and
Xavier Montagutelli)
Genetic monitoring of inbred strains using microsatellites.
 2) Electrophoresis and analysis of results

Wednesday 26th November

09:00 - 11:00

Lecture:
Genetic analysis of a heritable (Mendelian) trait - Analysis of inheritance –Genetic mapping
(Xavier Montagutelli)

11:00 - 11:30

Coffee break

11:30 - 13:00  

Practical session:
( supervised by Xavier Montagutelli, Jean-Jacques Panthier and Fernando Benavides.)
Genetic mapping of recessive mutation.

 13:00 - 14:00  

Lunch

  14:00 - 15:00  

15:00 - 17:30

Thursday 27th November

09:00 - 12:30

12:30 - 13:3

13:30 - 15:00

15:00 - 18:00   

Practical session:
( Supervised by Xavier Montagutelli, Jean-Jacques Panthier and Fernando Benavides)
Genetic mapping of a recessive mutation.

4) Electrophoresis - Analysis of the haplotypes. Fine mapping of the mutation.

Friday 28th November

09:00 - 10:30  

Lecture:
Keeping stocks segregating for a transgene.
Effects of the genetic background
(Xavier Montagutelli)

10:30 - 11:00

Coffee break

11:00 - 12:00

Lecture:
The Management of an animal breeding facility
(Fernando Benavides)

12:00 - 13:00 

Lunch

13:00 - 14:30

Lecture:
Mutagenesis in the Mouse and Rat
(Jean-Louis Guénet)

14:30 - 15:00

15:00 - 18:00

   Practical session:
(Supervised by Jean Jaubert)
Genetic databases mining via the internet

Monday 1st December

09:00 - 11:00

11:00 - 11:30  

Coffee break

11:30 - 12:30

Lecture:
Genetic analysis of hypertension in the rat
(Jose Eduardo Krieger)

12:30 - 13:30

Lunch

13:30 - 14:30

Lecture:
Embryo and sperm cryopreservation
(Jean Jaubert and Martina Crispo)

14:30 - 17:00

Practical session:
Embryo and sperm cryopreservation
(Jean Jaubert, Martina Crispo and Luiz Augusto Corrêa Passos)

Tuesday 2nd December

09:00 - 11:00

Practical session:
(Supervised by Xavier Montagutelli)
QTL detection in a mouse model of lupus erythematosus

11:00 - 11:30  

Coffee break

11:30 - 13:00  

Lecture:
Functional Genetics: Experimental Modification of the Mouse Genome
Classic transgenesis in mice
(Marcelo Rubinstein)

13:00 - 14:00 

    Lunch

14:00 - 17:30

Practical session:
(Supervised by Martina Crispo and Jean-Jacques Panthier) 
Dissection of Myf5^lacZ, Kit^lacZ and Dct^lacZ transgenic embryos at E9, E10, E11 and E12 - X gal staining

Wednesday 3rd December

09:00 - 12:30

Practical session:
(Supervised by Martina Crispo, Jean Jaubert and Luiz Augusto Corrêa Passos)
Manipulation of oocytes and early embryos: in vitro fertilization and culture, collection of fertilized embryos

12:30 - 13:30 

   Lunch

13:30 - 15:30

Lecture :
Functional Genetics: Experimental Modification of the Mouse Genome: Targeted mutagenesis
(Michel Cohen-Tannoudji)

15:30 - 16:00

   Coffee break

16:00 - 18:00

Practical session:
(Supervised by Mariela Bollati, Martina Crispo, Michel Cohen-Tannoudji and Jean Jaubert)
1) In vitro culture of fertilized eggs
2) ES cell culture
3) In vitro Cre mediated deletion using reporter cell lines

Thursday 4th December

09:00 - 12:00

Practical session:
(Supervised by Mariela Bollati, Martina Crispo and
Michel Cohen- Tannoudji)
1) ES cell culture
2) In vitro Cre mediated deletion using reporter cell lines.

13:00 - 14:00

  Lunch

14:00 - 16:00

Practical session:
(Supervised by Martina Crispo and Jean Jacques Panthier) Analysis of expression pattern in stained transgenic embryos.
In vitro manipulation of early mouse embryos

16:00 – 17:00  

Friday 5th December

09:00 – 10h30

10:30 - 11:00 

11:00 - 12:30

12:30 - 13:30  

13:30 - 17:30   

18:00 – 18:30        

20:00