Programme (pre)
Basic Microscopy, Fluorescense Microscopy, Confocal Microscopy, Selective Plane Illumination Microscopy, Spinning Disc Confocal Microscopy, Rapid Line Scanning Confocal Microscopy, Image Processing and Deconvolution, Imaging of model organisms, Förster resonance energy transfer (FRET), Fluorescence recovery after photobleaching (FRAP), Fluorescence lifetime imaging Microscopy (FLIM), Fluorescence correlation and cross-correlation spectroscopy (FCS and FCCS).
Day 1 |
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Monday June 18th |
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09.00-12.30 |
Student presentations |
16.00-18.00 |
Sample preparation |
Day 2 |
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09.00-12.30 |
Basic and Confocal Microscopy |
13.30-18.00 |
Basic microscopy practical |
Day 3 |
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09.00-12.30 |
FRAP, FRET, FLIM and FCS |
13.30-18.00 |
Confocal microscopy practical |
Day 4 |
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09.00-12.30 |
FRAP, FRET, FLIM and FCS |
13.30-18.00 |
F-techniques practicals |
Day 5 |
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09.00-12.30 |
In Vivo Imaging, 3-D Microscopy and SPIM |
13.30-18.00 |
F-techniques practicals |
Day 6 |
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09.00-13.00 |
In Vivo Imaging of Mice |
Sunday June 24th |
FREE |
Day 7 |
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09.00-12.30 |
3-D imaging |
13.30-18.00 |
3-D imaging practicals* |
Day 8 |
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09.00-12.30 |
3-D imaging |
13.30-18.00 |
3-D imaging practicals* |
Day 9 |
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09.00-12.30 |
Model systems |
13.30-18.00 |
3-D imaging practicals* |
Day 10 |
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09.00-12.30 |
Model systems |
13.30-18.00 |
3-D imaging practicals* R. Kraut, V. Korzh, D. Gilmour and S. Ahmed |
Day 11 |
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09.00-11.00 |
Student presentations |
11.00-12.00 |
Course review |
12.00-14.00 |
Lunch and course ends. |
*3-D Imaging practicals will feature:
Practicals will be run over two afternoons and students will be given a choice of two out of the four 3-D imaging systems. On the last day of the course students will present an overview of the 3-D imaging experiments. |
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