Theoretical

• design of fluorescent probes, from small molecules to quantum dots

• sample preparation

• visible fluorescent proteins and their applications

• basic fluorescence microscopy and spectroscopy

• latest developments in microscopy

• image processing

• tracking molecules in cells

• fluorescence lifetime imaging microscopy (FLIM)

• fluorescence resonance energy transfer (FRET)

• fluorescence recovery after photobleaching (FRAP)

• fluorescence correlation spectroscopy (FCS)

• atomic force microscopy (AFM) and force spectroscopy

Practical

• fluorescent labeling of biomolecules

• expression of VFP constructs

• staining and handling of biological samples

• basic and advanced (confocal, two-photon) fluorescence microscopy

• imaging protein reaction kinetics in cells by FRAP

• FRET imaging in living cells

• FLIM imaging in living cells

• tracking molecules in cells with quantum dots

• fluorescence correlation microscopy

• image processing

• atomic force microscopy of single molecules and cells

• force spectroscopy and mechanical properties of biomolecules