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"Advances in technologies for high resolution in vivo microscopy.
From single molecule detection to functional studies"


6th - 18th August | 2006 |Buenos Aires| Argentina

 

Preliminary Programme


Theoretical

  • design of fluorescent probes, from small molecules to quantum dots

  • sample preparation

  • visible fluorescent proteins and their applications

  • basic fluorescence microscopy and spectroscopy

  • latest developments in microscopy

  • image processing

  • tracking molecules in cells

  • fluorescence lifetime imaging microscopy (FLIM)

  • fluorescence resonance energy transfer (FRET)

  • fluorescence recovery after photobleaching (FRAP)

  • fluorescence correlation spectroscopy (FCS)

  • atomic force microscopy (AFM) and force spectroscopy


Practical

  • fluorescent labeling of biomolecules

  • expression of VFP constructs

  • staining and handling of biological samples

  • basic and advanced (confocal, two-photon) fluorescence microscopy

  • imaging protein reaction kinetics in cells by FRAP

  • FRET imaging in living cells

  • FLIM imaging in living cells

  • tracking molecules in cells with quantum dots

  • fluorescence correlation microscopy

  • image processing

  • atomic force microscopy of single molecules and cells

  • force spectroscopy and mechanical properties of biomolecules