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Genome, transcriptome and miRNome
analysis of single cells

27th Aug - 1st Sept| 2006 |Cologne| Germany

 

Programme (preliminary)

August 27th


August 28th


August 29th


August 30th


September 1st

 

August 27th

16.15 -19.00 Welcome & Dinner at 18.30

August 28th

09.00 - 12.00 Lecture 1
Single cell analysis
– an introduction Bosio, Cologne
Lecture 2
Magnetic cell sorting, Schmitz, Bergisch Gladbach
Lecture 3
Fluorescence activated cell sorting, Radbruch, Berlin
Lecture 4
Laser microdissection, Gangus,
Bernried
Lunch        
13.00 - 15:45 Practical 1
Magnetic cell sorting
(MACS) Assenmacher, Bergisch Gladbach
     
Break        
16.15 - 19.00 Practical 2
Fluorescence activated cell sorting (FACS), Göttlinger, Cologne
     
Dinner

 

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August 29th

09.00 - 12.00 Lecture 5
Cellular functioning of single neurons, Eberwine, Philadelphia
Lecture 6
Probe generation from small numbers of cells, C. Xiang, Bethesda
Lecture 7
Analysis of disseminated cancer cells, Klein, Munich
Lecture 8
Analysis of neural precursor cells, Cremer Marseille
Lunch        
13.00 - 15.45 Practical 3
Single cell amplification Part I, Biervert,  Bergisch Gladbach
     
Break        
16.15 - 19.00 Practical 4
Laser microdissection Part I, Baran, Bernried
     
Dinner

 

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August 30th
09.00 - 12.00 Lecture 9
Amplification using terminal continuation, Ginsberg, New York
Lecture 10
Analysis of T cell subsets, Appay, Lausanne
Lecture 11
Analysis of B cells, Kueppers, Essen
Practical 5
Single cell PCR – practical aspects, Kueppers, Essen
Lunch        
13.00 - 15.45 Practical 6
Single cell amplification Part II, Biervert, Bergisch Gladbach
     
Break Break      
16.15 - 19.00 Practical 7
Microarray Part I, Rüberg, Cologne
     
Dinner   top    
August 31st
09.00 - 12.00 Lecture 12
CGH using oligonucleotide arrays, Anderson, Palo Alto, USA
Lecture 13
CGH and Microdissection &Matix CGH, Joos, Heidelberg
Student talks (6) & Poster session
Lunch        
13.00 - 15.45 Practical 8Microarray Part II, Gerstmayer, Cologne      
Break        
16.15 - 19.00 Practical 9
Laser microdissection Part II, Baran, Bernried
     
Dinner   top    
September 1st
09.00 - 12.00 Lecture 14
Perspectives of miRNA analysis Pfeffer, Strasbourg
Practical 10
Practical approaches to miRNA analysis Pfeffer, Strasbourg
Practical 11
Microarray data analysis, Gerstmayer, Cologne
Lunch    
13.00 - 15.45 Practical 12
Microarray data interpretation,Hofmann, Cologne
 
Break    
16.15 - 19.00 Summary & Round Table Discussion top