Practical Course

European Molecular Biology Organization

 

Marine animal models in

evolution and development

 

 

 

 

 



12 - 23 July| 2010 |Fiskebackskil|Sweden

Programme (outline)

 

Sunday 11th

 

Arrival, evening lectures, Welcome dinner
Claus Nielsen: A guide through animal diversity
Max Telford: The New Animal Phylogeny

Monday 12th  to Tuesday 13th
 

Metazoan phylogeny: Sampling
Claus Nielsen, Harald Hausen, Andreas Hejnol

A full sampling day on metazoan diversity exploring the rich diversity of the fjord, harbouring almost all known animal phyla. Collection of specimens for cross-reactive antibody staining, injection and behavioural experiments, guided by the interest of the students.

Tuesday 13th to Wednesday 14th
 

Environmental genomics and phylogeny
Andreas Hejnol, Max Telford

A course module on bioinformatics, teaching and discussing latest strategies in environmental genomics and phylogeny reconstruction

Wednesday 14th to Friday 16th
 

Reverse genetics and transgenesis.
Detlev Arendt, Ina Arnone, Michalis Averof, Evelyn Houliston, Zacharias Kontarakis, Ulrich Technau

Microinjection techniques. Delivery of dsRNA/siRNA, morpholinos, or expression constructs into Parhyale, Platynereis, Planarian, Nematostella and sea urchin embryos. Students are encouraged to transfer techniques to their organism of choice.
Transgenesis of, Parhyale, Platynereis, and sea urchin, using different transformation systems (piggyBac, Minos transposon systems).

Saturday 17th to Sunday 18th
 

Paleontological field trip
Graham Budd, Alison Daley

Monday 19th to Thursday 22nd
 

In situ stainings using cross-reactive reagents
Ina Arnone, Detlev Arendt, Michalis Averof, Evelyn Houliston, Gaspar Jekely

In situ hybridization and immunolocalization techniques in, Platynereis, Strongylocentrotus, Paracentrotus. Cross-reactive reagents will be tried on embryos and larvae collected in the field and/or brought by the participants.

Wednesday 21st to Thursday 22nd
 

Behavioural studies in marine zooplankton
Detlev Arendt, Gaspar Jekely

In this module we will look at swimming behaviour of different marine plankton, including ciliated larvae of annelids and cnidarians. We will record swimming trajectories, measure swimming speed and the efficiency of phototaxis. We will also test the effect of different neurotransmitter agonists and antagonists on these behaviours.

Friday 23rd
 

All students and teachers

Student presentations and discussions.

Course wrapup.

Party

Saturday 24th
 

Departure

 

 

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