About the Practical Course
This practical course focuses on single-molecule methods for the nanomanipulation of individual biomolecules such as DNA and the study of protein-DNA interactions.
Single-molecule nanomanipulation approaches provide unparalleled access to key structural and kinetic features of complex biochemical reactions involving DNA, for instance in its transcription, replication and repair. As single-molecule methods for studying molecular interactions in real-time become more robust and user-friendly, their implementation is observed to spread from specialized biophysics research groups to the general biological research space.
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Topics
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Construction of a magnetic trap |
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Construction of an optical tweezer |
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Introduction to software issues |
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Sample preparation |
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Mechanical properties of nucleic acids |
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Analysis of transcription, replication and repair machineries |
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Introduction to data analysis |
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This two-week course is intended to provide graduate students, postdoctoral fellows and independent researchers from the fields of biochemistry and protein chemistry with an experimental foundation for performing such experiments with either custom-built or commercial instrumentation.
As only 16 students can be admitted to this year’s course, priority will be given to applicants demonstrating a short- to medium-term need for these methods in their research projects.
We look forward to seeing you in Paris! The Organizers |
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