Programme (FINAL)
UPDATE: DOWNLOAD LATEST PROGRAMME (word.doc)
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Our Practical Course has a busy hands-on programme. Each day starts with a lecture, followed by practical work until lunch. Lunch is followed by more practical laboratory work after lunch, until 5 pm, when students can join special interest groups. These groups provide the opportunity for the participants to get more skilled in specific procedures and discuss their work with experts. Two afternoons will be dedicated to the presentations of the participants’ research. |
Time |
Saturday, Sep 11 |
Sunday, Sep 12 |
Monday, Sep 13 |
Tuesday, Sep 14 |
Wednesday, Sep 15 |
08:30 - 09:30 |
Arrival and registration
Welcome and Introduction |
Lecture: S. Volarević, Regulation of the p53 tumour suppressor by ribosomal proteins |
Lecture: N. Dear, Forward and reverse genetic approaches to elucidating gene function in the mouse |
Lecture: I. Kalajzic, Use of transgenic models to understand differentiation of mesenchymal stem cells |
Lecture: H. Takayanagi, Mouse genetics in osteoimmunology |
09:30 - 10:00 |
Break |
Break |
Break |
Break |
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10:00 - 11:00 |
Groups AB – 2C: Mouse handling and dissection, basic behavioural phenotyping (V. Katavić) |
Group A – 4K: Early mouse development (M. Mallo) Group B – 4A: Open cardiac surgery (I. Grković ) |
Group B – 4K: Early mouse development (M. Mallo) Group A – 4A: Open cardiac surgery (I. Grković ) |
Groups AB – 2C: Mouse anatomy, basic surgical procedures (V. Katavić) |
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11:00 - 12:00 |
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12:00 - 13:00 |
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13:00 - 14:00 |
Lunch |
Lunch |
Lunch |
Lunch |
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14:00 - 15:00 |
Participants’ presentations
Sightseeing and Dinner |
Lecture: I. Dikic, Mouse models in ubiquitin networks – relevance to human diseases |
Group A – 4A: Visual markers of mesenchymal differentiation (I. Kalajzic) Group B – 4K: Late mouse development – Skeletal staining of mouse embryos (M. Mallo) |
Group A: Late mouse development – Skeletal staining of mouse embryos (M. Mallo) Group B: Visual markers of mesenchymal differentiation (I. Kalajzic) |
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15:00 - 16:00 |
Break |
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16:00 - 17:00 |
Lecture: A. Marušić, Anatomy of the mouse |
Participants’ presentations |
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17:00 - 18:30 |
Lecture: M. Mallo, Old Hox genes playing new tricks to build the mouse skeleton |
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18:30 - 19:30 |
Dinner |
Dinner |
Dinner |
Dinner |
Time |
Thursday, Sep 16 |
Friday, Sep 17 |
Saturday, Sep 18 |
Sunday, Sep 19 |
08:30 - 09:30 |
Lecture: I. Barbaric, Embryonic stem cells/induced pluripotent stem cells - tools for developmental studies, disease modelling and regenerative medicine |
Lecture: B. Polić, The role of activating and inhibitory receptors in the development and effector functions of NK cells |
Lecture: D. Grčević, What can we learn from mouse models of human diseases? |
Round Table Discussion
Best presentation award |
09:30 - 10:00 |
Break |
Break |
Break |
Break |
10:00 - 11:00 |
Group A – 4A: Cryosections: Standard staining and immuno-hystochemistry (M. Heffer-Lauc) Group B – 4K: Colon cancer models: tissue collection and analysis (J. Terzić) |
Group A – 4K: Isolation of blastocysts and embryo transfer (B. Polić) Group B – 4A: Cryosections: Standard staining and immuno-hystochemistry (M. Heffer-Lauc) |
Group B – 4K: Isolation of blastocysts and embryo transfer (B. Polić) Group A – 4H: Demonstration – spinal nerve ligation (D. Sapunar) Special Interest Groups |
Questionnaires and Discussion
Groups AB – 4K/2C: Special Interest Groups
Departure/Lunch |
11:00 - 12:00 |
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12:00 - 13:00 |
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13:00 - 14:00 |
Lunch |
Lunch |
Lunch |
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14:00 - 15:00 |
Group A – 4K: Colon cancer models: tissue collection and analysis (J. Terzić) Group B – 4A: Surgical approach to CNS (M. Heffer-Lauc) |
Group A – 4A: Surgical approach to CNS (M. Heffer-Lauc) Group B – 4H: Demonstration – spinal nerve ligation (D. Sapunar) Special Interest Groups |
Groups AB – 4K/2C: Special Interest Groups |
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15:00 - 16:00 |
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16:00 - 17:00 |
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17:00 - 18:30 |
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18:30 - 19:30 |
Dinner |
Dinner |
Dinner |
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Ivana Barbaric, Sheffield, UK: Embryonic stem cells/induced pluripotent stem cells - tools for developmental studies, disease modelling and regenerative medicine |
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Neil Dear, Leeds, UK: Forward and reverse genetic approaches to elucidating gene function in the mouse |
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Ivan Đikić, Frankfurt, Germany: Mouse models in ubiquitin networks – relevance to human diseases |
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Danka Grčević, Zagreb, Croatia: What can we learn from mouse models of human diseases? |
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Ivo Kalajzić, Farmington, CT, USA: Use of transgenic models to understand differentiation of mesenchymal stem cells |
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Moises Mallo, Oeiras, Portugal: Old Hox genes playing new tricks to build the mouse skeleton |
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Bojan Polić, Rijeka, Croatia: The role of activating and inhibitory receptors in the development and effector functions of NK cells |
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Hiroshi Takanayagi, Tokyo, Japan: Mouse genetics in osteoimmunology |
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Siniša Volarević, Rijeka, Croatia: Regulation of the p53 tumor suppressor by ribosomal proteins |
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A special lecture will be devoted to responsible research involving laboratory animals, including the 3R principles of animal research. |
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Isolation of embryos E8.5, 9.5 and 11.5 (including isolation of yolk sac for genotyping) Staging of embryo’s age Basic histochemical staining techniques for embryos – bone, LacZ. Analgaesia and surgery and in mice Bleeding – tail vein and terminal cardiac or retroorbital puncture Terminal formaldehyde perfusion Default necropsy and identifying major organs Cryosectioning Injection - i.v. (tail vein), s.c., i.m., i.p. Basic surgical procedures in mice Isolation and analysis of important cell populations, e.g. bone marrow, culturing fibroblasts from skin Adoptive transfer of hematopoietic cells Visualizing of GFP-tagged tissues from transgenic mice Xenografts – i.v., s.c., into flanks |
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