Practical Course

European Molecular Biology Organization

 

Ubiquitin and SUMO

 

 

 

 

 



29 July - 4 August | 2010 | Split |Croatia

Programme (preliminary)

 

Presentations by Participants:

At the beginning of the course, all participants will present their research projects in short (10 min) talks.

Poster Sessions:

All participants will present their research on a poster, which will be used as a point of reference for discussion of specific questions, feedback and input from the instructors and the other participants.

Discussions:

Informal discussion sessions (“beach discussions”) will be held daily at the local beach. Every day at least two instructors or guest speakers will be available to discuss projects and experiments or background issues with the participants. This format as proven particularly effective and enjoyable during previous courses.

   

1. Non-covalent interactions of ubiquitin and ubiquitin-like proteins (Dikic)

- affinities of different ubiquitin binding domains for monoubiquitin and polyubiquitin chains of different linkages
- comparison of binding specificities among ubiquitin, SUMO and Nedd8
 

2. In vitro analysis of the ubiquitin/proteasome system (Joazeiro)

- characterisation of RING finger ubiquitin ligases
- degradation of ubiquitinated proteins by the proteasome
   

3. In vitro sumoylation assays (Melchior)

- in vitro sumoylation assays using bacterially expressed enzymes
- discussion of purification strategies
- influence of enzyme concentrations, E3 ligases and SUMO orthologs
- participants have the option of analysing their own samples
   

4. In vivo ubiquitylation assays (Polo)

- enrichment of ubiquitinated species in vivo
- detection of ubiquitin-dependent endocytosis by immunofluorescence
 

5. Detection of ubiquitin and SUMO targets in yeast (Ulrich)

  

- target isolation and detection under denaturing conditions
- variations in target protein, modifier, detection strategy and tagging
- participants have the option of analysing their own samples
   

6. A bioinformatical approach to the ubiquitin system (Hofmann)

- general methods for similariy searches, alignments, domain content analysis and other analysis tools
- prediction of protein modification and ubiquitin recognition
- assignment of ubiquitin-relevant domains and identification of new homology domains.

 


  

 

  

 

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