About the Practical Course
BACKGROUND:
The regulatory influence exerted by members of the ubiquitin family pervades virtually all areas of cellular biology; yet, their analysis is often difficult and requires specialised knowledge. For example, isolation and in vivo analysis of ubiquitin and SUMO substrates is complicated by the presence of deconjugating enzymes whose activities need to be overcome.
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Strategies for identification of modification sites or modifying enzymes vary depending on the type of target, and in vitro analysis requires proper isolation and handling of the relevant enzymes.
Our course is aimed at students and postdocs in all areas of cellular biology who have encountered the ubiquitin and/or SUMO system in the context of their specific biological question and wish to address the significance of their findings. |
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COURSE OBJECTIVES: |
• to present state-of-the-art methodology for the practical analysis of ubiquitylation and sumoylation
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• to introduce newcomers to the field with the basic concepts of the ubiquitin and SUMO systems
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• to provide background knowledge about the application of proteomics, mass spectrometry and genomic screening approaches to the ubiquitin and SUMO systems
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• to provide the opportunity for participants to bring their own samples for analysis
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We will especially select those students/postdocs who come from laboratories with limited experience in ubiquitin/SUMO research but are working on projects that are expected to benefit from the acquisition of expertise in this methodology. Preference will be given to students and postdocs in relatively early stages of their projects, as this is expected to provide maximal return for their own research and their labs.
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Although the main emphasis will be on practical work, lectures by leading scientists in the field, poster sessions and informal discussions will give participants the opportunity to learn about functions of the ubiquitin and SUMO system in various areas of cell biology and to obtain input into their specific projects from experts and colleagues.
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We look forward to welcoming you to Split!
The Organisers |
Co-Sponsors |
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