Practical Course

European Molecular Biology Organization

Monday 31st May
Morning	Expression Strategies
	Bacterial expression Baculovirus expression Mammalian cell expression HT techniques
Afternoon	Practicals
Evening	Student Poster session

Tuesday 1st June
Morning	Isolation of Complexes
	Purification of recombinant multi-subunit omplexes Purification of complexes from natural sources TAP- and DARPin technology Characterizing Protein/DNA complexes
Afternoon	Practicals
Evening	Special lecture

Wednesday 2nd June
Morning	Complex Characterization
	SAXS/SANS Large-scale studies of complexes Protein-protein interaction databases
Afternoon	Practicals
Evening	Student Poster session

Thursday 3rd June
Morning	Use of EM & NMR
	Single-Particle methods Tomography NMR of complexes Intrinsically unstructured proteins
Afternoon	Practicals
Evening	Special lecture

Friday 4th June
Morning	Complementary techniques
	Yeast 2-hybrid methods Mass spectrometry of complexes Chemical biology to probe interactions Fluorescence microscopy imaging
Afternoon	Practicals
Evening	Course Banquet

Saturday 5th June
Morning
	Case Studies of Challenging Projects Exploiting latest developments at the ESRF