Programme (preliminary)
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L = lecture P = practicals D = demonstration E = theoretical exercise |
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Day 1 |
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15:30 |
Student presentations; 7 min each |
20:00 |
Get-Together of Students and Teachers; Welcome party |
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Day 2 |
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09:00 |
Introduction (L) |
09:15 |
Overview of course techniques (L) |
09:45 |
Principles of transmission electron microscopy (L) |
10:30 |
Break |
11:00 |
Chemical fixation (L) |
12:00 |
Lunch |
13:00 |
Introduction to plastic embedding and sectioning (L) |
14:00 |
Introduction to cryo sectioning (L) |
16:00 |
Introduction and practical details of immunolabelling (L) |
17:00 |
Split students into small groups and rotate the groups between the different labstations (lab-tour, TEM, cryo-ultramicrotomes, ultramicrotomes, microwave, knife-makers, freeze-substitution) 15 min at each station (D) |
19:30 |
Dinner followed by informal poster session |
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Day 3 |
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09:00 |
Vitrification of water (L) |
10:00 |
Sampling, rapid freezing and cryoprocessing (L) |
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(high pressure freezing and freeze substitution) |
10:45 |
Break |
11:15 |
Colloidal gold and its applications (L) |
12:00 |
Split students into Groups A and B (10 in each) |
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Group A – Cryo sectioning/labelling |
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Group B – Plastic embedding/sectioning/negative staining |
12:30 |
Lunch |
13:30 |
Practicals (open end) |
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Day 4 |
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09:00 |
Theory and pitfalls of immunolabelling (L) |
09:45 |
Correlation between EM and Light microscopy (L) |
10:30 |
Break |
11:00 |
Collodial gold particle labelling for EM (L) |
11:45 |
Counting gold particles and relating them to cell compartments (L) |
12:30 |
Lunch |
13:30 |
Practicals |
19:30 |
Social event |
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Day 5 |
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09:00 |
Latest developments in cryo sectioning (L) |
09:45 |
Interpretation in EM (L) |
10:30 |
Break |
11:00 |
General discussion and rotation of groups A and B |
12:30 |
Lunch |
13:30 |
Practicals (open end) |
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Day 6 |
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09:00 |
Quantitative TEM (stereological tools) (L) |
10:00 |
Break |
10:30 |
New methods for quantifying gold labelling (L) |
11:30 |
Numbers, length, areas (E) |
12.30 |
Lunch |
13:30 |
Quantification of colloidal gold (E) |
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Touring Oslo in the afternoon |
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Day 7 |
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09:00 |
EM tomography |
10:45 |
Cryo EM and high resolution tomography |
11:30 |
Break |
12:30 |
General Discussion |
14:30 |
Practicals (open end) |
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Day 8 |
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09:00 |
Practicals |
12:30 |
Lunch |
13:30 |
Practicals |
19:00 |
Farewell party |
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Day 9 |
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09:00 |
Practicals |
13:00 |
Lunch |
14:00 |
Final Discussion |
15:00 |
Some departures |
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Day 10 |
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Some more departures |
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