Registration; Introduction
Basic culture techniques; transfections;
Gene targeting- basic principles
Gene targeting- vector design & construction
Databases and resources
Round table: individual projects (with instructors)
Introduction to culturing DT40 cells
Basic protocol I: Maintenance, transfection/ plating of DT40s.
Basic protocol II: Preparation of genomic DNA.
Basic protocol III: FACS analysis of DT40 cell cycle

Gene targeting- tackling essential genes; tagging
Cell cycle analysis: checkpoints, etc.
Cell cycle analysis: synchronisation of DT40 cells
Round table: individual projects (with instructors)
Basic protocol I: Maintenance, transfection/ plating of DT40s; picking/ expanding colonies
Basic protocol II: Analysis of genomic DNA- digestion.
Basic protocol III: 2d FACS analysis of DT40 cell cycle

Approaches to microscopy of DT40s
Chicken karyotype analysis; SCEs
Analysis of signalling/ apoptosis in DT40s
Basic protocol I: Maintenance, transfection/ plating of DT40s
Basic protocol II: Analysis of genomic DNA- Southern gel/ transfer
Metaphase spreads of DT40 cells
Basic protocol IV: fluorescence microscopy of DT40 cells; Elutriation/ synchronisation of DT40 cells;
Analysis of signalling/ apoptosis in DT40s

Repair/ recombination assays; telomere analysis
Chemical genetics and proteomic approaches
Basic protocol II: Analysis of genomic DNA- probe testing/ (cold) hybridisation
Basic protocol I: Maintenance of DT40 cells.
Basic protocol IV: fluorescence microscopy of DT40 cells; Elutriation/ synchronisation of DT40 cells;
Analysis of signalling/ apoptosis in DT40s

Microarray analysis/ chromosome engineering/ other
Basic protocol I: Maintenance of DT40 cells.
Basic protocol II: Analysis of genomic DNA- (cold) Southern result
Basic protocol IV: fluorescence microscopy of DT40 cells; Elutriation/ synchronisation of DT40 cells;
Analysis of signalling/ apoptosis in DT40s
Completion/ writeup
Review and Discussion - individual projects