About the Practical Course
DT40 is a transformed chicken B-cell line that has the particular property of allowing very high ratios of targeted to random integration. This permits gene targeting with very high efficiency, a notable plus in designing robust genetic experiments.
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The cells are quite easy to work with and relative novices to cell culture can make rapid progress in reverse genetic analyses in this system. |
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This course will allow people to assess whether this system can contribute to their research questions. After completing the course, participants should be able to carry out a DT40 gene targeting experiment and to perform the basic phenotypic analysis of their targeted cells. Additional objectives lie in informing participants of more advanced techniques and approaches available in the model system. click image below to view/download poster |
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This course should be of interest to researchers who have limited experience of gene targeting and tissue culture, possibly those considering moving from another model system into vertebrate cell culture models. As the DT40 system is relatively cheap, it is possible that this approach may interest start-up laboratories or labs with moderate resources. We expect that this course will be of interest to PhD students/ early stage postdocs. Ideally, the participants will have a 'favourite gene' that they are thinking about analysing in DT40, or an idea that they want to explore in the system.
We look forward to welcoming you to Galway! The Organisers
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