Practical Course

European Molecular Biology Organization

Transient kinetic methods

applied to biological

macromolecules

 

 

 

 

 



27 June - 3 July | 2010 | Canterbury| United Kingdom

Programme (summary and preliminary programme)

 

The course will last for 6 days (27 June - 3rd July, 2010 ) from Sunday evening to departure on Saturday and will follow the pattern of

the previous EMBO courses.

In summary:

Each full day will consist of 3 hours of lectures 4.5 hours of hands-on practical work and finish with a 1 hour evaluation/feedback tutorial.

The first two full days will be a structured course aimed at familiarising the participants with the methods and equipment to be used.

The remaining time will be structured around the interests of the participants and will include studies of more advanced equipment and methods and studies of the participants’ own samples and data.

Each student will be assigned a personal tutor for the week (3 students per tutor) who will be responsible for contacting the student prior to the course, to discuss what experiments will be possible in the time available and to design a programme of study for each student.

The week will begin on Sunday evening with short presentations by the participants of their background and the experimental systems in which they are interested.

Friday afternoon will be taken up with the presentation by the participants of the results of the studies they have undertaken during the week.

 

Sunday 27th

 

 

17:00-18:00

REGISTRATION

 

18:00-19:00

BUFFET – Howard Rogers Room

 

19:00-21:00

Welcome

Introduction to the course including 5 min. presentation by participants

 

 

 

 

Monday 28th

BLT1

 

09:00

Introduction to Transient Kinetics

R.S. Goody

09:40

Introduction to Stopped Flow

M.A. Geeves

10:20

Introduction to Quench Flow

C. Lionne

11:00

COFFEE – Howard Rogers Room

11:20

Introduction to Data Analysis

J. Reinstein

12:00

Meeting with Practical tutors

 

12:30

LUNCH – Rutherford College

13:15-15:00

Practical 1 (see schedule)

 

15:30

COFFEE – Howard Rogers Room

15:50

Practical 2 (see schedule)

 

18:00

Group Tutorial – BLT1

 

19:30

DINNER

 

 

 

Tuesday 29th

BLT1

 

09:00

Stopped Flow – Examples

R.S. Goody

09:40

Data Analysis/Modelling

J. Reinstein

10:20

Quench Flow – examples

C. Lionne

11:00

COFFEE – Howard Rogers Room

11:20

Optics for rapid-reaction equipment

C. Bagshaw

12:30

LUNCH – Rutherford College

13:15

Practical 1 (see schedule)

 

15:30

COFFEE – Howard Rogers Room

15:50

Practical 2 (see schedule)

 

18:00

Group Tutorial – BLT1

 

19:30

DINNER

 

 

 

Wednesday 30th

BLT1

 

09:00

Probing interaction kinetics by solid phase detection techniques

J. Piehler

09:40

Flash photolysis

I. Chizhov

10:20

Relaxation methods – Theoretical background

D. Pörschke

11:00

COFFEE  – Howard Rogers Room                                       

11:20

Single molecule kinetics

C. Bagshaw

12:00

Meeting with Tutors

 

12:30

LUNCH – Rutherford College

13:15-18:00

Students own studies, pressure jump and flash photolysis

 

15:30

COFFEE – Howard Rogers Room

18:00

Group Tutorial – BLT1

 

19:30

DINNER

 

 

 

Thursday 1st July

BLT1

 

Morning

 

 

09:00

Relaxation methods – examples

D. Pörschke

09:40

Fluorescence anisotropy applied to stopped flow measurements

J. Eccleston

10:20

Probing complex protein-protein interactions on membranes by multi-parameter solid phase detection

J. Piehler

11:00

COFFEE – Howard Rogers Room

11.20

Pressure Jump

M. Geeves

12:30

LUNCH – Rutherford College

 

 

 

 

 

 

Afternoon

 

 

13:15-18:00

Students own studies

 

15:30

COFFEE – Howard Rogers Room

18:00

Group Tutorial

 

 

 

 

Friday 2nd

To be arranged from the following

 

Morning

  1. Students complete their own studies and prepare materials for final presentation

 

 

  1. Practical demonstrations on systems not used during the week

 

11:00

COFFEE – Howard Rogers Room

12:30

LUNCH – Rutherford College

 

 

 

Afternoon

BLT1

 

13:30-17:30

Feed back session
15 min. presentations by students of work during the week

 

15:15

COFFEE – Howard Rogers Room

19:00

CONFERENCE DINNER

 

 

 

 

Saturday 3rd

Departure

 

 

 

 

 

 

 



 

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