Practical Course

European Molecular Biology Organization

 

PHOSPHOPROTEOMICS

 

 

 

 

 



28 March - 2nd April | 2010 | Odense| Denmark

About the Practical Course

 

Phosphoproteomics is a relatively new field, which aims at characterizing all phosphorylated proteins in a cell or tissue at a given time.

Phosphorylation is participating in almost all cellular processes where proteins are tightly controlled by reversible phosphate groups that are reversibly linked to the proteins by enzymes (kinases/phosphatases).

 
 
In order to understand such processes in detail the discovery of phosphorylation sites involved in such biological events has to be identified.  Thus, efficient methods for characterization of phosphorylated proteins are needed. Many of such methods are previously described with various success and output.

The phosphoproteomics course focuses on the practical issues concerning phosphoproteomics which is of utmost importance in being able to characterize phosphorylated proteins. It is aimed at scientists (cell biologists, bio-/protein-chemists and others) who have an interestt in cellular regulation with phosphorylation or who are heading towards implementing phosphoproteomic strategies in their research project.

 

In the course we will describe new strategies for phosphoproteomics, extensive sample handling and purification steps for phosphopeptides together with data interpretation strategies.

The organizer of this course has been in the area of phosphoproteomics since 1996. He has made a significant methodological contribution to the area by inventing numerous methods for assessment of phosphorylation sites and enrichment of phosphorylated peptides ((Titanium dioxide chromatography: Larsen MR et al., Mol Cell Proteomics 2005, 4(7), 873-86; and SIMAC: Thingholm TE et al., Mol Cell Proteomics. 2008, 7(4), 661-71)).

 

Recently we have been exploring strategies for large scale phosphoproteomics which will also be included in the course.

We look forward to welcoming you to Odense!

 

The Organisers

Co-Sponsor


 

European Molecular Biology Organization | Meyerhofstrasse 1 | 69117 Heidelberg | Germany