Programme (overview)
The practical course will be concerned with the following topics: |
|
|---|---|
| • | The use and pitfalls of detergent extraction |
| • | The preparation of plasma membrane vesicle and spheres for phase separation studies |
| • | Giant unilamelar vesicles of model membranes |
| • | Lipid protein interactions using reconstituted liposomes and giant unilamelar vesicles |
| • | Measurement of membrane order employing c-laurdan |
| • | Lipid analysis by mass spectrometry |
| • | Florescence correlation- and STED microscopy |
| • | Fluorescent lipids for cell biology |
