Practical Course

European Molecular Biology Organization

Lipid rafts:

methods for studying

membrane organization

 

 

 

 

 



23 - 31st May | 2010 | Dresden| Germany

Programme (overview)

 

The practical course will be concerned with the following topics:

   

The use and pitfalls of detergent extraction

The preparation of plasma membrane vesicle and spheres for phase separation studies

Giant unilamelar vesicles of model membranes

Lipid protein interactions using reconstituted liposomes and giant unilamelar vesicles

Measurement of membrane order employing c-laurdan

Lipid analysis by mass spectrometry

Florescence correlation- and STED microscopy

Fluorescent lipids for cell biology

 

 

 

 

 

European Molecular Biology Organization | Meyerhofstrasse 1 | 69117 Heidelberg | Germany