Practical Course

European Molecular Biology Organization

High throughput methods for protein
production and crystallization



17 - 25 June | 2009 |Oxford | United Kingdom

Programme

 

Wednesday  17th June

 

Introduction: Ray Owens

 

CLONING AND EXPRESSION

Lecture: Nick Berrow

High throughput construction of expression vectors

 

Lecture: Renaud Vincentelli

High throughput expression screening and refolding in E. coli

 

Coffee break

 

Lecture: Yves Durocher

Mammalian cell expression - theory and practice

 

Lunch

 

Practical session: (course instructors)

 

Poster session at WTCHG ( with dinner buffet and drinks)

 

Thursday 18th June

 

Lecture: Ian Jones

Insect cell expression - theory and practice

 

PROTEIN PRODUCTION AND CRYSTALLIZATION

Lecture: Joanne Nettleship

High throughput protein purification and characterization

 

Coffee break

 

Lecture: Karl Harlos

The OPPF crystallisation facility

 

Lunch

 

Practical session: (course instructors)

 

 Dinner

Friday  19th June

 

Visit to Diamond Light Source

Lecture and tour : Liz Duke

Data collection at synchrotrons

 

Lunch

 

Practical session: (course instructors)

 

Student talks

 

 Dinner

 

Saturday 20th  June

 

Practical session: (course instructors)

 

Lunch

 

Practical session: (course instructors)

 

Dinner

 

Sunday 21st  June

 

FREE DAY

 

Monday 22nd June

 

BIOINFORMATICS

Lecture: Ian Overton

 

Coffee break

 

Workshop: Jon Diprose

 

Lunch

 

Practical session: (course instructors)

 

Student talks

 

 Dinner

 

Tuesday 23rd June

 

COMPLEMENTARY METHODS

Lecture: Robert Gilbert

Electron microscopy

 

Coffee break

 

Lecture: Carol Robinson

Mass spectrometry

 

Lunch

 

Practical session: (course instructors)

 

Student talks

 

 Dinner

 

Wednesday  24th June

 

Practical session: (course instructors)

 

Lunch

 

Practical session: (course instructors)

 

Conference Dinner

 

Thursday  25th June

 

Practical session and roundtable : (course instructors)

 

Lunch

 

DEPARTURE



 

 

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European Molecular Biology Organisation (EMBO) | Meyerhofstrasse 1 | 69126 Heidelberg | Germany