Programme (outline)
10-day preliminary headlines:
Students will be divided into four groups of four.
Two groups will spend the first week on magnetic trapping and the second week on optical trapping while the other two groups will
proceed in the opposite order.
Lectures will be combined with hands-on experiments, computer exercises and demonstrations.
Magnetic Trapping
-Assembly of the prototypical magnetic trap
-Preparation of DNA samples and surfaces for nanomanipulation
-Force calibrations, signal-to-noise considerations
-Mechanical response of DNA to twisting and stretching
-Assaying DNAzymes: RNA polymerases, topoisomerases and helicases
-Demonstration of commercial instruments
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Optical Tweezers
-Basic optics (laser safety; beam characterization; beam alignment; lenses, mirrors, telescopes, etc.)
-Hands-on experience with a functional dumbbell optical trap.
-Disassembly and reassembly of a functional dumbbell optical trap (extremely succinctly: upstream: beam characterization, alignment and steering; beam injection; downstream: beam detection)
-Trap characterization, signal-to-noise considerations
-Position-clamp mode
-Active force-clamp mode ; passive force-clamp mode (to be confirmed)
-Assaying RNA polymerase
-Assaying myosin or kinesin motor proteins (to be confirmed)
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