Practical Course

European Molecular Biology Organization


ADVANCED OPTICAL MICROSCOPY

 

 

 

 

 



25 March - 4th April | 2009 |Plymouth |United Kingdom

About the Course

There will be 2- 3 lectures each day throughout. The first 3 days will be spent setting up microscopes for brightfield and epifluorescence microscopy, including polarisation and interference contrast methods. 

 

The final 6 days will be organised in rotations around the 9 experiments listed below. The experiments will be in the large classroom at the MBA and in rooms nearby.

 

Use will be made of marine specimens where possible and student may have an opportunity to trawl for benthos and plankton, depending on weather and availability of the collecting boat.

Developmental Biology

1,2  two experiments with confocal imaging
3    CCD imaging and deconvolution, ,microinjection,
genetically encoded probes, gene gun

Cell Physiology
4        Confocal imaging with electrophysiology
       CCD imaging with electrophysiology
       Photolysis and photometry
7        Total internal reflection fluorescence imaging
       Laser traps
9        Marine bioluminescence

10      Multiphoton imaging with both LaVision and single-spot Zeiss systems

 

 

Imaging Optics

- Quantitative interference microscopy

- Use of an optical bench for basic experiments on scanning and apertures

- Spectroscopy and filters

- Abbe’s experiments on the role of diffraction in microscope image formation with absorption and phase objects.

- Demonstration of plasmon resonance imaging of live cells


Preparations
used will include: invertebrate preparations (tunicates, molluscan neurones), zebra fish, mammalian brain slices, Fucus, Arabidopsis, and cell lines.

Special demonstrations and hands-on exercises

   

We look forward to seeing you in Plymouth!

The Organisers

     

 

 

 

European Molecular Biology Organisation (EMBO) | Meyerhofstrasse 1 | 69126 Heidelberg | Germany