Practical Course

European Molecular Biology Organization


ELECTRON TOMOGRAPHY


IN LIFE SCIENCE

 

 

 

 

 



1 - 6 June | 2009 |Leiden |The Netherlands

About the Course

 

ONLINE QUESTIONNAIRE

*** PLEASE COMPLETE THIS CONFIDENTIAL QUESTIONNAIRE AT THE END OF THE COURSE.

Click HERE to access it. Thank you!

 

Electron tomography is a 3D-imaging technique with a resolution range bridging the gap between optical imaging and single particles 3DEM techniques.

 
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This six-day course will be given at the Leiden University Medical Center, the Netherlands and will cover the major aspects of:

electron tomography

specimen preparation,

data acquisition,

alignment

tomogram visualization

interpretation

The course aims to train junior investigators in electron tomography techniques to carry out 3D imaging and reconstruction of biological samples in their home institute.


The participants will be introduced to the required specimen preparation steps for high-resolution electron tomography.

Techniques that will be discussed include cryofixation, freeze-substitution and cryosectioning.

 

Different approaches and software packages will be used to acquire (dual-axis) tomographic tilt-series and to align tilt series.

 

Visualization of acquired tomograms will also be discussed and practiced. Throughout the course lectures will be given by experts in the field together with practicals on all covered aspects.

 

We invite PhD students and post-doctoral fellows with a life sciences background interested in 3D imaging of biological specimens, such as tissue, cells, organelles and macromolecular complexes to participate in the course.

We look forward to seeing you in Leiden

The Organizers

   

 

 

 

European Molecular Biology Organisation (EMBO) | Meyerhofstrasse 1 | 69126 Heidelberg | Germany