Practical Course

European Molecular Biology Organization


MASS SPECTROMETRY & PROTEOMICS

 

 

 

 

 



15 - 22 April | 2009 |Odense| Denmark

About the Course

 

*** PLEASE COMPLETE THIS CONFIDENTIAL QUESTIONNAIRE AT THE END OF THE COURSE.

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The course focuses on mass spectrometry analysis of proteins and peptides in the rapidly developing field of proteomics.

Mass spectrometry offers a world of opportunities for protein analysis, especially when combined with advanced separation techniques and bioinformatics.

New methods for sample handling, protein and peptide enrichment procedures, fragmentation techniques, and data interpretation tools are popping up every day and it is difficult to comprehend all the exciting possibilities in current proteomics research. More accurate, sensitive and advanced mass spectrometers are developed and the interpretation of all the data requires background knowledge and experience.

Topics

  1. Peptide and protein analysis and by MALDI-MS and MS/MS technologies, including sample preparation.

  2. Protein analysis and peptide sequencing by ESI-MS and LC-MS/MS.

  3. New developments in MS/MS fragmentation of peptides, e.g. ETD and ECD.

  4. Analysis of post-translational modifications by MS, including phosphopeptide enrichment techniques.

  5. MS-based quantification strategies within Proteomics

  6. Computational data-analysis, including database searches, de novo sequencing, data validation and bioinformatics analysis of large datasets. 

This EMBO practical course in Mass spectrometry and Proteomics aims to provide an overview of state-of-art techniques for the analysis and characterization of protein and peptide samples by ESI and MALDI Mass Spectrometry and tandem mass spectrometry (MS/MS), as well as analysis of post translational modifications, such as acetylation, glycosylation and phosphorylation.

The course will provide the participants with hands-on experience on mass spectrometry analysis and associated data analysis.

The course is focused on practical issues in Mass Spectrometry driven Proteomics, and it is aimed at cell biologists, bio-/protein-chemists, bioinformaticians and others with an interest in modern mass spectrometry for protein and peptide analysis in Proteomics.

Co-Sponsor