Programme
This five-day training programme will provide (to the 20 selected attendees) an opportunity to utilise leading-edge tissue microarray This intensive practical course will incorporate specialized seminars, demonstrations and laboratory work, with both wet-lab and computer-based activities being envisaged.
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1. |
Tissue processing and paraffin-embedded block construction. |
2. |
Tissue microarray construction and sectioning. |
3. |
Immunohistochemical staining, with emphasis on application of automated approaches. |
4. |
Digital slide scanning of both full face section and tissue microarray slides. |
5. |
Data storage and use of online approaches for digital slide management. |
6. |
Image analysis: manual and automated methods. |
7. |
Use of novel tools to support complex digital slide visualisation tasks. |
8. |
Construction of protein arrays. |
9. |
Standard protein array protocols and associated image analysis. |
Target group |
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construction and image analysis facilities as well as protein array technology available at the UCD Conway Institute,Ireland.