About the Practical Course

The objective of this course is to teach a wide range of state of the art methods for the in vitro and in vivo functional analysis of ubiquitylation and sumoylation.

Step by step protocols will be covered in lectures and practical experiments regarding questions such as:

i)	is the protein of interest modified in vivo/in vitro;
ii)	which E2 / E3 enzyme is involved;
iii)	how can one identify the modification site;
iv)	does modification alter stability, localisation or interactions.

Practical experiments include, e.g., in vitro and in vivo modifiation reactions, analysis of non-covalent interactions with ubiquitin and SUMO, enrichment of modified species from cells, and a short course on bioinformatic tools for the prediction of modification sites, recognition motifs and interacing partners. Some experiments will allow researchers to bring their own samples, e.g., purified protein for in vitro sumoylation.
Lectures will cover a wider range of methods that can be applied to study SUMO and ubiquitin in diverse cellular processes including, e.g., endocytosis, signal transduction, nuclear transport, and DNA repair. Designated times for informal discussions as well as shared meals will provide ample opportunity for interactions with the teachers and instructors.

Practical experiments include, e.g., in vitro and in vivo modifiation reactions, analysis of non-covalent interactions with ubiquitin and SUMO, enrichment of modified species from cells, and a short course on bioinformatic tools for the prediction of modification sites, recognition motifs and interacing partners.

Some experiments will allow researchers to bring their own samples, e.g., purified protein for in vitro sumoylation.

Lectures will cover a wider range of methods that can be applied to study SUMO and ubiquitin in diverse cellular processes including, e.g., endocytosis, signal transduction, nuclear transport, and DNA repair.

Designated times for informal discussions as well as shared meals will provide ample opportunity for interactions with the teachers and instructors.

Co-Sponsor

About the Practical Course