Programme (outline)
** Updated: Course Schedule (pdf )
This is an intensive course with seminars in advanced topics with top-researchers in the field, demonstrations from equipment vendors as well as extensive practical sessions where the students will develop their projects and use the available equipment.
Topics addressed in seminars:
Microscopy, /windows to development, Fluorescence & GFP, Multidimensional Imaging; Confocal and Multiphoton Microscopy, OPT; SPIM & MIAM, MicroMRI/CT imaging, OpenMicroscopy environment and Image-data management, Analysis of cell movement and tissue morphogenesis, 3D Image Processing and analysis, Publication of 3D images/movies
The practicals will include:
Embryo/tissue culture and preparation, Fluorescence stereoscopes, confocal and multiphoton microscopes, widefield+deconvolution systems, OPT, automated image acquisition, 3D image reconstruction and analysis software (free and commercial).
Five different projects will be planned and distributed to 5 groups (covering as many different models as possible) which will be distributed according to technical needs and equipment availability.
The equipment will either be run by the staff or students (preferably) and there will be senior scientists available to provide scientific and technical support constantly.
After image acquisition, the students will be trained in image management tools, image processing, and in using software for 3D image analysis and morphometrics.
In the end the students will learn techniques to display and publish 3D images/movies, and will prepare a mini-talk as well as a short poster with their observations/results.