About the Practical Course

3D imaging in developmental biology is currently a topic of major interest

(the number of articles referenced “3D development imaging” in pubmed which totaled 129 before 1999, has increased steadily to an average of more than 40 /year in the last 3 years).

*** Deadlline for applications: May 8th, 2008

While molecular biology techniques evolved fast and became routinary in the last decades, interest in 3D microscopy imaging started mostly after the popularization of the confocal microscope in the 90’s, and is now gaining increasing interest with the fast pace of development of new imaging techniques. These are specifically addressing the limited depth of field and deteriorated axial resolution of optical microscopes, which facilitate the acquisition of detailed images of cell behavior inside whole embryos (MPLSM; SPIM, OPT; microMRI; OCT etc...).
Visualization, processing and analysis of multidimensional images is also now facilitated thanks to the development of high-end PC and graphics processign units, as well as the apprearance of free and commercial 3D image analysis software. This practical course is focused on addressing all these aspects of 3D imaging, with special emphasis on their application to the study of Developmental Biology.
This 8 day practical course is aimed at young researchers in the Developmental Biology research field, interested in answering specific questions that require observation of cell movement and tissue morphogenesis in whole embryos, which are particularly difficult using conventional optical microscopy techniques. Participants will have the opportunity to learn and test state-of-the-art techniques/protocols for imaging whole embryos (both fixed and live), and are expected to apply the techniques to their experimental model of choice, obtain high quality 3D images, learn to manage, process and analyse multi-dimensional data-sets, and learn strategies for publishing 3D images/movies.	a) Click here to: View original poster b) Click here to: Download print version

While molecular biology techniques evolved fast and became routinary in the last decades, interest in 3D microscopy imaging started mostly after the popularization of the confocal microscope in the 90’s, and is now gaining increasing interest with the fast pace of development of new imaging techniques.

These are specifically addressing the limited depth of field and deteriorated axial resolution of optical microscopes, which facilitate the acquisition of detailed images of cell behavior inside whole embryos (MPLSM; SPIM, OPT; microMRI; OCT etc...).

Visualization, processing and analysis of multidimensional images is also now facilitated thanks to the development of high-end PC and graphics processign units, as well as the apprearance of free and commercial 3D image analysis software.

This practical course is focused on addressing all these aspects of 3D imaging, with special emphasis on their application to the study of Developmental Biology.

This 8 day practical course is aimed at young researchers in the Developmental Biology research field, interested in answering specific questions that require observation of cell movement and tissue morphogenesis in whole embryos, which are particularly difficult using conventional optical microscopy techniques.

Participants will have the opportunity to learn and test state-of-the-art techniques/protocols for imaging whole embryos (both fixed and live), and are expected to apply the techniques to their experimental model of choice, obtain high quality 3D images, learn to manage, process and analyse multi-dimensional data-sets, and learn strategies for publishing 3D images/movies.

a) Click here to: View original poster
b) Click here to: Download print version

Co-Sponsors

About the Practical Course