Programme (pre)
This five-day training programme will provide (to the 20 selected attendees) an opportunity to utilise leading-edge tissue microarray construction and image analysis facilities available at the UCD Conway Institute, Ireland. This intensive practical course will incorporate specialised seminars, demonstrations and laboratory work, with both wet-lab and computer-based activities being envisaged.
The training will be primarily delivered by a group of 9 expert researchers from the University College Dublin, Royal College of Surgeons in Ireland/Beaumont Hospital, Dublin City University, US National Cancer Institute and Lund University.
Seminars will be combined with wet-lab and computer-based practical sessions in tissue microarray technology covering the following aspects:
1) Tissue processing and paraffin-embedded block construction.
2) Tissue microarray construction and sectioning.
3) Immunohistochemical staining, with emphasis on application of automated approaches.
4) Digital slide scanning of both full face section and tissue microarray slides.
5) Data storage and use of online approaches for digital slide management.
6) Image analysis: manual and automated methods.
7) Use of novel tools to support complex digital slide visualisation tasks.
Target group
Potential users of tissue microarrays, who come from fields such as molecular biology, biochemistry, cell biology, developmental biology, (molecular) cytogenetics, (molecular) pathology, etc. Participants with either basic research interests or applied interests (medicine, agriculture, industrial biotechnology, etc.) are welcome. We do not expect that participants in the Practical Course have had formal training in physics, engineering, or computer science.
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