Programme

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The course will be structured so as to interleave lectures and practical sessions. The core of the course will be followed by all participants, although towards the end of the course there will be the possibility of selecting a topic from a number of specialist modules, such as thermafluor studies of protein stability and mass spectroscopy as a routine tool for quality assessment.

The core activity will cover the process of HTP protein production from target selection (bioinformatics) through parallel cloning (96 well format), parallel protein expression screening (both E. coli and eukaryotic cells), protein expression scale-up and purification and crystallization. Participants will be encouraged (via a pro-active organization prior to the course) to bring their own targets, which they will be able to enter into the process at any of these points.

Where possible work will follow the flow of actual experiments, although to cover the full range of activities, some aspects will be run off-line (for instance bioinformatics and target selection will be covered whilst the cloning experiments are on-going).

The course will therefore cover the following:-

Target selection / LIMS (lecture + practical)

HTP cloning + expression screening (lecture + practicals)

HTP baculovirus/mammalian expression construction (lecture + practical)

Treatment of glycosylated proteins (lecture)

Library-based methods (lecture)

HTP protein purification and characterization (lecture +practical)

HTP protein crystallization (lectures + practical)

Specialist modules chosen from several including mass spectroscopy and thermofluor (lecture + practical)