High-throughput (HTP) sequencing of eukaryotic, viral and bacterial genomes is providing a huge database of proteins of possible biomedical relevance for structure-function analysis. In response to this opportunity, structural proteomics projects have been initiated world-wide with the aim of establishing HTP structure determination on a genome-wide scale. Crucial to this effort has been the development of HTP technologies for the protein production (cloning, expression purification), protein crystallization and improved methods for structure solution by X-ray crystallography.

The aim of this course will be to review the state-of-the-art of high-throughput (HTP) protein production and crystallization methods in structural biology with an emphasis on using these methods to study complex and ‘difficult’ targets and their rescue. Practical training will be provided in HTP methods for cloning, protein expression screening using both E. coli and eukaryotic systems, protein purification and crystallization. Participants will be able to apply the high-throughput protein production and crystallization methods to their own protein target(s) with the option of entering their target(s) into the process  at various stages.