| Invited Teachers and Instructors |
Gareth Griffiths (European Molecular Biology Laboratory, Heidelberg, Germany)
has broad experience in the application of EM immunocytochemistry, EM and stereology for analysis of the biosynthetic, endocytic and phagocytic pathways. |
| Paul Webster (House Ear Institute, Los Angeles, USA)
has been a regular teacher in our courses for over 15 years and is a specialist in a wide range of EM techniques who has a lot of experience in immunocytochemical applications. He also has a broad expertise in anatomy and histology. Paul is a superb teacher and has organized many courses for immuno electronmicroscopy in the USA. In recent years he has played an increasingly important role, along with Prof. Hagler, in organising the online course manual and website that in part overlaps with his own excellent website in Los Angeles. |
Heinz Schwarz
(Max-Planck Institute, Tübingen).
has a wide experience in immunolabelling cryo and resin sections for both LM and EM. He has been a regular teacher on these courses for 15 years. He has also organized his own annual EM three-day course in Tübingen for 20 years. He is an absolute compendium of knowledge about anything to do with EM.
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Herb Hagler (UT Southwestern Medical Center) A superb engineer who has designed many EM tools including the cryo chamber of one of the two commercially available cryo microtomes. Has also contributed greatly to improving glass knives for sectioning. Has also a broad experience in immunocytochemical applications for pathology. He is a master of fixing just about any machine related to our course. With 20 participants this can become a full time job but it cannot be emphasized enough how dependent we are, not only on the availability of the best available technology, but also on it running smoothly. He is also a computer expert and, along with Paul Webster, is responsible for putting the course manual on the web. |
John Lucocq (University of Dundee, UK)
has a broad expertise in applying EM techniques for studying membrane trafficking events. He is also a stereology specialist who has developed new methods for relating the numbers of gold particles to cell surfaces and volumes. He has extensive experience with the use and application of gold colloids. Also here he has made pioneering insights. He has been a member of the teaching team for over 10 years. |
Peter J. Peters (Netherlands Cancer Institute, Amsterdam) has extensive expertise in applying immuno-EM techniques for investigating membrane traffic in cells and how this may relate to disease states. The focus is on understanding the molecular machinery and organization of molecular sorting within the endomembrane system of a cell. Cryo immunogold-electron microscopical methods are the main techniques, which allow the subcellular detection of gene products at the highest resolution. His passion is to improve the ultrathin cryosectioning for high-resolution 3D tomography. In the last 5 years he co-developed with Diatome several cryo-diamond (trimming) knifes. He is a regular on the course since 1998. |
Terry Mayhew (University of Nottingham, UK) In our view he is the best teacher of stereology in the world who has a fantastic didactic ability to reveal the simplicity in what is usually considered as a difficult subject. He combines his lectures with simple practical examples where the students are given micrographs and, transparent lined lattices. They are shown how easy it is to estimate surfaces, volumes, lengths and numbers of organelles from selected examples and are required to estimate these parameters. He has been a member of the teaching team for 12 years and this interaction has recently stimulated the development of a new method for quantifying gold distributions in organelle compartments and on cell membrane surfaces.,
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Local teaching team |
Norbert Roos (University of Oslo) has a broad experience in cryo techniques for EM. He developed a novel method for combining cryo EM with immunolabelling (Roos et al, EMBO J. 1996). He has organized about 12 courses in Oslo, the first in 1985. He has been a regular teacher on these courses. In this course we plan to give more experienced (or very keen) students the opportunity to learn the vitrified grid cryo EM approach for isolated particles as well as the labelling method developed by Roos. Cryo EM will not be a main focus of this course but the equipment and expertise is available to allow some participants to learn some aspects of it on a case by case basis. |
Espen Stang (senior scientist at the National Hospital) is well trained in various aspects of immunoEM will be responsible for the operation of cryo-utramicrotomes and immunolabelling.
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Andreas Brech (senior scientist and head of EM unit at the National Cancer Hospital)
is well trained in various aspects of immunoEM will be responsible for the operation of cryo-utramicrotomes and immunolabelling. |
Ketil Pedersen (postdoc at the National Hospital)
is well trained in various aspects of immunoEM will be responsible for the operation of cryo-utramicrotomes and immunolabelling. |
Nicolas Baroisis (postdoc at the Dept. of Molecular Biosciences)
is also well trained in various aspects of immunoEM will be responsible for the operation of cryo-utramicrotomes and immunolabelling. |
Randi Olsen (senior technician at the EM-unit, University of Tromsø)
with experience in a wide range of electron microscopy techniques |
Torill Rolfsen & Tove Bakar (senior technicians)
have together over 60 years of experience in almost all aspects of electron microscopy |